Journal of Zoological and Botanical Gardens

Swimming is an important behavior for all penguin species. However, zoo-housed penguins typically do not swim as often as their wild counterparts, which may have consequences for their health and welfare. In an effort to increase the swimming time of the African penguin (Spheniscus demersus) population at Lincoln Park Zoo in Chicago, IL, USA (21 adults: 13 males, 8 females), we introduced a commercially available bubble curtain to the outdoor pool within the penguins’ habitat. The bubble curtain pushes pressurized air out through a hose fitted with small holes to create a stream of bubbles that generate water movement, which could entice penguins to swim. Over the course of 2 months, the penguins were exposed to a series of alternating conditions characterized by the bubble curtain being off or on for 2-week periods. A total of 228 swimming bouts were observed during this study. The bubble curtain did not increase the amount of time the penguins spent swimming, nor the maximum number of penguins in the pool during swim bouts. Rather, the penguins spent more time swimming when the bubble curtain was turned off, and the number of penguins in the pool during swim bouts was consistent across experimental phases. Additionally, we found that penguins swam the most when air temperatures were between 31 and 40 °F (approximately −1 to −4 °C). Unexpectedly, at least three individual penguins swam overnight between the hours of midnight and 6:00, highlighting the value of monitoring animals during entire 24 h periods. Collectively, this study provides detailed information about the swimming behavior of a zoo-housed African penguin population, and indicates that a bubble curtain was ineffective at stimulating swimming. Full article

Measurement of zoo animal welfare states enables improvement to husbandry and management to be evidence-based and implemented according to species’ needs. Theoretical welfare concepts are often discussed, and whilst it is helpful to ensure wide consensus across all stakeholders on what welfare comprises, practical application of such evidence-based information is as equally important. All species housed in zoos will have specific needs that must be met by their housing, husbandry, and care to enable them to thrive. Therefore, this paper examined how to identify key animal care needs and an animal’s responses to them to form a basis for species-specific welfare assessment approaches. There are examples of familiar-to-the-zoo species that still pose challenges regarding delivery of optimal husbandry and management. As such, the identification and evaluation of core concepts of the biology, behaviour, “needs and wants” of these species is required to support validation and refinement of physical, behavioural, and psychological welfare measures. This article evaluated the use of evidence to build capacity in welfare measurement for a familiar zoo-housed species, the giraffe (Giraffa camelopardalis, Linnaeus 1758), by outlining seven key needs and requirements that must be provided in the zoo (“giraffe W-E-L-F-A-R-E” = Warmth, Enrichment, Leaves, Feeding, Alfalfa, Rumination, Exercise). Provision of these inputs, and opportunities for all giraffes in a herd to engage with them, provides the foundation for further welfare assessment to be implemented. Specifically, the validation and measurement of mental states that are more likely to be positive if key behavioural and ecological needs have already been met. This paper advocated for this evidence-based approach to “welfare-focussed husbandry”, with distillation of key information that supports species-relevant care, to be developed for other zoo-housed species as support for their welfare assessment protocols. Such welfare-focussed husbandry is layered on top of the basic animal care requirements of the species in the zoo to ensure all individuals have the best opportunity to attain positive welfare states. In this way, and once validated, foundational welfare assessment can be easily completed by busy animal care staff, capacity is built into zoo operations as all stakeholders are aware of exactly what each species needs, and deeper dive welfare assessment (especially concerning animal mental states) can be targeted more effectively. Further evolution of these seven steps for the giraffe is suggested and extrapolation of this approach, to aid identification of key welfare indicators across all zoo-housed species, is encouraged. Full article

Knowledge about ecological conditions and processes in centers of endemism (CoEs) is still limited with respect to various systematic groups of organisms, ecosystem types, ecological conditions, and ecosystem services. We review the characterization, identification, and meaning of CoEs. Endemics play an increasing and prominent role in nature conservation monitoring and management and in the organization of zoos, aquaria, and botanical gardens. We examine the importance of different groups of organisms and indicators for the characterization of endemic-rich regions, e.g., with regard to the richness of endemics per region and degree of endemism, the importance of heterogeneity in space, continuity in time, isolation, and ex situ management for the survival of endemic species. Currently, conversion of land cover and land use change are the most important causes of biodiversity decline and extinction risk of endemic and endangered species. These are followed by climate change, including severe weather, and then natural processes such as volcanism, landslides, or tsunamis. For conservation purposes, the management of regional land use, zoos, aquaria, botanical gardens, and social aspects of the diversity of endemics and CoEs have to be taken into account as well. We find that the ex situ representation of endemics in general is limited, and conservation networks in this regard can be improved. We need better answers to questions about the relationship between ecoregions, CoEs and regional awareness of endemism, which is linked with human culture including aesthetics, well-being, health, and trade. Full article

The COVID-19 pandemic forced many educators out of their traditional settings and into web-based learning environments. Zoos were no different, and throughout the pandemic they strived to reach their supporters using different approaches. By adapting quickly and using outreach activities and online learning, zoos continued to serve society through the pandemic. This study examined whether an in-person educational interaction with zoological specimens can enhance cognitive learning and increase primary-level students’ attitudes towards animals and science. Additionally, attention was drawn to the effectiveness of online learning methods for young pupils. A total of 165 paired questionnaires from primary-level students in three schools were analysed. Findings indicate significant positive increases in attitude but not in learning achievements. Thus, zoological specimens can be utilised for enhancing scientific attitudes in primary-level students. The effect of a face-to-face lesson on learning compared with online methods was positive but the difference was not statistically significant. This, taken together with a significant positive effect of a face-to-face lesson on attitude improvements, is sufficient to determine the importance of a traditional learning environment for students of this educational level. Additionally, gender differences relating to scientific attitudes and understanding are not apparent at primary level but this may become more apparent at secondary level. This research may be used for further investigations into the relationship between age, gender, and scientific learning. It may also be used to support studies examining the effectiveness of zoo outreach programs in schools versus zoo visits. Full article

Scientific research is the main driver to push forward and disseminate botanical knowledge. Despite many institutions having this fundamental aim as a core activity, many of them do not have a complete set of facilities, expertise, staff, and resources to cover all the steps involved in the study, management, conservation, and popularization of plant diversity. Accordingly, we propose a workflow formalizing the cooperation between a botanical garden and a botanical research center, focused on the study of plant endemic species. Specifically, the cooperation was implemented between the PLANTSEED Lab of the Department of Biology and the Botanic Garden and Museum of the University of Pisa. We present seven representative case studies (Armeria arenaria complex, Bellevalia webbiana, Crocus etruscus and C. ilvensis, Dianthus virgineus complex, Pulmonaria hirta complex, and Santolina chamaecyparissus complex) to disentangle the approaches and opportunities arising from cooperative approaches, from laboratory to cultivation. We analyze the emerging properties derived from this synergistic cooperation by promoting open research questions and answering them using a comprehensive approach to improving endemic plant species research, conservation, and popularization in the botanical garden. In this manuscript, we show how a cooperative approach between heterogeneous botanical institutions can constitute an effective and easy-to-implement approach to achieve the goals of each partner involved in the cooperation. Full article

One of the main goals of zoos and aquariums (hereafter ‘zoos’) is to connect visitors with animals. Unfortunately, COVID-19 lockdowns resulted in these organizations closing around the globe, making this goal exceedingly difficult. During lockdowns, zoos became very resourceful and found alternative means to connect people with animals. Additional social media, webcams, and education resources were offered. What is unknown, however, is the extent to which people used these resources and what factors influenced this. This study, therefore, aimed to explore these questions through an online survey. Of the 302 participants who chose to stay connected to a zoo, the majority (82%) did so via social media, and just over half of the sample used webcams (51%). When asked why they stayed connected, 75% indicated that they did so for their own interest in animals, but some (36%) said they enjoyed sharing the animal information with family/friends. Zoo members were more likely to use education resources, and people with zoo work experience were more likely to share animal information. This study illustrates that zoo resources were utilized during lockdown and that demographic variables were associated with how and why people used them. The findings have implications for zoos post-COVID-19 and may be useful for promoting connections and well-being in certain cohorts of society. Full article

Botanic gardens play an increasingly important role in the conservation of global biodiversity. However, although botanical gardens periodically report the results of introducing certain species of native flora, they rarely attempt to summarize existing knowledge to make general recommendations regarding ex situ collections. The aim of this study was to analyze the many years of experience of the Tashkent Botanical Garden in creating and maintaining living collections of threatened species of Uzbekistan (the majority of which are endemic to the country or Central Asia) in order to identify species whose cultivation ex situ is advisable, and whose cultivation will not result in meaningful conservation. Careful analysis of the species introduction history revealed that a simple dichotomy of the introduction results (success/failure) appears to be an oversimplification. In terms of the cultivation success, the introduced plant species can rather be classified into three categories: success, failure, and dubious success. For many species whose introduction was earlier considered successful, the introduction success is questionable and further efforts to conserve these species ex situ should be abandoned. A decision tree and classification of threatened perennials for possible ex situ introduction are proposed and the species in TBG collections are tabulated according to the latter. Species considered unsuitable for ex situ conservation are recommended for quasi in situ conservation. Both approaches, ex situ and quasi in situ, should be intensively used as a part of an integral conservation strategy for preserving plant biodiversity. Full article

It is now well established that human-induced species extinctions and habitat degradation are currently occurring at unprecedented rates. To halt and reverse this decline, the international community adopted the Kunming–Montreal Global Biodiversity Framework (GBF), as part of the Kunming–Montreal Biodiversity Package, in December 2022. We clarify what this new framework means for conservation zoos and aquariums in their mission to prevent species extinction by highlighting areas of focus. We explain why it is necessary that conservation zoos and aquariums establish the appropriate mechanisms for contributing towards such a framework to help validate their role in the 21st Century. Conservation zoos and aquariums should be reassured that much of their work already fits within the GBF. However, the current mechanisms for individual zoos and aquariums to directly contribute to the implementation of the GBF mostly rely on close collaboration with individual national governments and/or are only possible at a national level. It is therefore critical that national, regional, and global zoo membership organisations take a leading role in championing the work of their members. Equally, adequately linking the efforts of zoos and aquariums to the national implementation of international instruments, such as the GBF, is imperative to ensure that these organizations’ contributions feed into the understanding we have of global progress towards the implementation of international instruments. Full article

The field of wildlife conservation is comprised of a variety of players with different contexts and approaches. Zoos and aquariums, historically largely focused on public entertainment, are shifting more towards conservation-minded missions and can play a unique role in wildlife conservation by leveraging their distinct assets. The Minnesota Zoo is an AZA-accredited institution and an agency of the State of Minnesota that has been conducting wildlife conservation for over 40 years. Here, we review our current portfolio of local field projects, including initiatives targeting pollinators, native mussels, turtles, and bison, using several considerations to structure and better understand how our unique context has shaped our work. Our designation as a state agency has impacted our initiatives by necessitating a focus on local efforts and has facilitated many partnerships with other government agencies. Indeed, partnerships have been vital to our success and have shaped our programs significantly since their inception. All of the Zoo’s conservation initiatives are built on a bedrock of sound science, and we continue to contribute to the field through research, utilizing the expertise of department staff. In addition, the various funding streams that support our programs have dramatically shaped our work and have created some siloing of staff within the department. However, grant funding can serve as a buffer against the impacts of economic uncertainty, as evidenced during the COVID-19 pandemic. Lastly, our programs have expanded and our objectives have pivoted over the years in response to changing needs and opportunities; such flexibility—and increased flexibility for our staff—is imperative to the future success of these efforts. The Minnesota Zoo’s narrative is unique and helps us understand how we can continue to most effectively carry out local conservation efforts. As we work to protect habitats and save species from extinction, it is important to utilize the distinct assets that each organization can contribute in order to have the greatest collective impact. Full article

This study explored Toronto Zoo animal-care professionals’ (ACPs) experiences with compassion fatigue (CF) using a two-phase participatory methodology. In phase one, 11 participants took photographs of their workplace. In phase two, participants told the story behind their photographs through one-on-one interviews. The data were analyzed using NVivo12 software. The participants’ experiences with compassion fatigue stemmed from issues with foundational infrastructure at the Toronto Zoo. Specifically, the participants highlighted issues related to training, staffing, and resource availability and discussed their resultant effect on animal welfare. The participants described the importance of built and sustained trust in their jobs, both with each other and with the non-human animals under their care. While the Zoo’s motto is “One TZ”, the participants noted conflict between the public’s perception of the Toronto Zoo and how the organization cares for its staff. The additive effects of mental and physical exhaustion have led to disengagement from activities that once brought joy and difficulty staying focused while at work and home. The findings will enable the Toronto Zoo to provide comprehensive mental health support for their staff and allow participants, researchers, partner organizations, and the general public to discover more about compassion fatigue in the hope that the lessons learned will last a lifetime. Full article

Rhinoceros are among the largest and most endangered herbivores in the world. Three of the five extant species are critically endangered, with poaching and habitat loss contributing heavily to declines. The gut microbiome is an essential facet of host health and digestion, mediating a variety of immune and physiological functions. Certain microbes have the potential to serve as biomarkers for reproductive outcomes and predictors of disease susceptibility. Therefore, assessing gut microbial dynamics in relation to wild and managed rhinoceros populations has particular relevance for zoos and other conservation organizations that maintain assurance populations of these charismatic megafauna. The functional gut microbiomes associated with all rhinoceros species remain poorly studied, and many published reports are limited by small sample sizes and sex biases. In this review, we synthesize current research to examine the rhinoceros gut microbiome under human management and resulting conservation implications, address common pitfalls of wildlife gut microbiome studies, and propose future avenues of research in this field. Full article

Euphorbia resinifera (Zaggûm or Tikiut) is an endemic species of Morocco that grows in the Middle Atlas Mountain range. It is used in folk medicine to treat several diseases, especially various types of cancer. Aim of the review: In this review, we critically highlighted and discussed previous reports on E. resinifera, concerning its botanical description, taxonomy, geographical distribution, and medicinal use. In addition, bioactive compounds, toxicology, and pharmacological effects were reported. Materials and methods: We searched various scientific databases, such as Scopus, PubMed, Web of Science, SpringerLink, SciFinder, Wiley Online, and Google Scholar, to collect data on E. resinifera. Studies involving E. resinifera or its bioactive compounds with regards to antitumor, anti-inflammatory, antileishmanial, antiprotease, immunomodulatory, irritant, and lysosomal activities are discussed here. Results: E. resinifera has been widely used in folk medicine practice in Morocco to treat several diseases such as diabetes, cancer, and inflammatory skin conditions, as well as to heal wounds. In phytochemistry studies, biochemical compounds have been identified from E. resinifera belonging specially to terpenoids. Indeed, in vitro and in vivo pharmacological investigations showed that extracts and compounds from the latex of E. resinifera exhibited a wide spectrum of biological properties, particularly antioxidant, antimicrobial, antitumor, anti-inflammatory, antileishmanial, and immunomodulatory and neuroprotective activities. Conclusion: The use of E. resinifera in conventional medicine is supported by processes founded on biological evidence. However, in-depth research is necessary to prove the safety and efficacy of E. resinifera latex extracts and their compounds and to clarify their pharmacological mechanisms. In addition, pharmacokinetic and pharmacodynamics studies are required for extracts and bioactive compounds. Full article

Research studies that shed light on cognitive and perceptual abilities in otters can utilize tasks that provide environmental, structural, food-based, sensory, or cognitive enrichment. The current study examined the use of the novel object recognition task, a task commonly used to study memory in non-human animals, as a form of sensory enrichment. The subject of the current study was an adult male otter that resided at the Seneca Park Zoo in Rochester, NY, USA. The stimulus pairs for this task were multisensory (3D objects and odorants). In this study, three memory intervals were investigated: 10 min, 1 h, and 24 h (each memory interval included 10 sessions). The otter spent only 15% of his time near the stimulus pairs and engaged in very few explorations, suggesting that this was not an effective form of sensory enrichment and did not provide any evidence for long-term memory. These results contrast strongly with our previous studies with otters using a two-alternative forced-choice task that provided engaging cognitive enrichment. We suggest that cognitive enrichment, including enrichment via training (and food-based enrichment), may be more effective for otters than sensory enrichment. Future research should further investigate cognitive phenomena in otters using tasks involving cognitive enrichment. These types of studies can improve enrichment practices and promote positive welfare for otters in zoos, inform conservation efforts, and grow our limited knowledge of otter perception and cognition. Full article

Although rare and threatened species are maintained in many botanical gardens around the world, detailed reports on the success or failure of their introduction appear infrequently, which makes it difficult to understand the major constraints of growing imperilled species in botanical garden living collections. Though intuitively, a level of endemism appears to be important, its role as a predictor of species cultivation success in the garden living collections has never been tested. This paper summarizes the experience of the Tashkent Botanical Garden in creating and maintaining living collections of rare and threatened species of Uzbekistan, trying to understand the role of endemism and other factors in the success and failure of these species cultivation. We found that out of 100 rare and threatened species introduced, the cultivation of 26 failed. Most of these species were endemic to the country, occupying soil types and habitats different from those of the garden site. However, surprisingly, the introduction of many analogous species has been successful. This implies that some narrow endemics can be successfully grown in botanical gardens, but to predict which can and which cannot is impossible, and there are no alternatives to introduction trials. Overall, the large number of rare and threatened species for which introductions were successful confirms the important role of ex situ conservation in preserving critically endangered biodiversity and should stimulate further work in this direction. The future efforts of the garden staff will focus on two major objectives: (i) collecting seeds of endangered species that have so far skipped attention or their collection missions have not been successful; and (ii) propagating those species that have proven cultivation success and using the propagated material for in situ actions. Full article

Given the current and future threats to Asian elephants (Elephas maximus), maintaining a sustainable ex situ population is crucial for the longevity of the species. Using Infrared Thermography (IRT), thermoregulation of Asian elephants at low ambient temperatures was examined. Thermal images were taken at 15 min intervals over 60–90-min observation periods, once weekly, during January and February 2022. A total of 374 images were examined from 10 Asian elephants, which varied from 1 to 56 years of age. Data from thermograms of the ear and body were interpreted in view of weight, age and behavior. Variability in surface temperature was found most frequently in the ears, occasionally presenting as thermal windows—areas with dense underlying blood supply that aid in heat exchange. Thermal windows occurred most frequently in the distal, then medial, regions of the ear. The pattern of appearance of thermal windows in the ear provides support that the increase of blood flow is utilized as a method of warming. This preliminary study provides key insight into Asian elephant thermoregulation, suggesting that the species may be more well-adapted to lower ambient temperatures than previously thought. Full article

In response to rapid biodiversity losses in recent decades, zoos have become more engaged in conservation issues. Solutions to conservation challenges are complex and require collaborative efforts across organizations. Zoos can be effective partners that can contribute diverse expertise and resources to protect wildlife and their habitats. While zoos often partner with international organizations to facilitate field-based conservation projects on the exotic animals they exhibit, some of the most meaningful conservation and education initiatives are conducted locally in partnership with local organizations. A core part of the mission of the North Carolina Zoo (Asheboro, NC, USA) is the conservation of wildlife and their natural habitats, both regionally and internationally. The goal of this article is to review the North Carolina Zoo’s regional conservation programs and the importance of partnerships with other local organizations in accomplishing shared goals. North Carolina Zoo plays an important role in regional conservation by protecting and managing natural lands, protecting declining amphibians through headstarting and habitat management, rehabilitating native wildlife, and working on local outreach and sustainability projects to reduce impacts on natural resources and inspire others to get involved in conservation. These programs were developed through partnerships with local and state government agencies, academic institutions, non-profit organizations, other zoos and aquariums, schools, libraries, and businesses. These collaborations have been instrumental in developing and implementing successful projects by pooling limited resources and sharing crucial expertise. They demonstrate how zoos are evolving to become leaders and partners in conservation, research, and education to protect local species and natural resources. Full article

Scientific research has long been recognized as one of the four pillars of the zoo or aquarium mission, alongside recreation, conservation, and education. This study sought to quantify a sample of zoos’ participation in scientific research via the provision of biomaterials from animals to outside scientists and the associated training of undergraduate and graduate students that resulted from these projects. A convenience sample of zoos provided data on their participation in biomaterials-related projects and a focused analysis of biomaterials-related research facilitated by the Saint Louis Zoo was conducted. In addition, the Association of Zoos & Aquariums’ conservation & science database was queried to uncover what research projects AZA members engaged in over the last five years that likely involved biomaterials research and training of students. Results demonstrate that zoos are making significant contributions to science by agreeing to participate in large numbers of biomaterials-related projects across different areas of focus involving a variety of animal species annually, with results applicable to human and non-human animals. Support of such research often involves student thesis projects, thus contributing to the education of future scientists. Full article

This communication discusses the Bear Care Group’s “behavior-based” enrichment philosophy, focusing on the importance of enrichment programs built on the knowledge of species’ natural histories, living strategies, and observed daily and seasonal routines. Methods for program development are discussed, detailing the benefits to animal wellbeing, including the reduction of chronic stress and frustration that lead to abnormal or stereotypical behaviors. The concepts are illustrated through a discussion of bear natural history and living strategies, but these methods are applicable to a wide range of taxa. The Bear Care Group encourages facilities to consider behavior-based enrichment programs to promote positive welfare for their animals. Full article